Nagoya University, School of Engineering Lecture information system (SYLLABUS)

Chemistry and Biotechnology Laboratory 3(3.0 credits)

Code:12443
Course Type:Specialized Courses
Class Format:Experiment
Course Name : Chemistry
Starts 1 : 4 Autumn Semester
Elective/Compulsory : Compulsory
Lecturer : Faculty of Chemistry  

•Course Purpose
This course enhances the development of the student's skill in carrying out organic chemistry and biotechnology experiments. It will help your graduation research in 4th grade (graduation thesis A and B). Through organic chemistry laboratory, you will learn advanced experimental protocols for the synthesis, separation, purification, and identification of organic molecules. Biotechnology laboratory aims to learn advanced experimental procedure.

•Prerequisite Subjects
Elemental Chemistry I & II, Organic chemistry 1 ~ 5, Biochemistry 1, chemistry and biotechnology laboratory 2.

•Course Topics
* Advanced organic chemistry
1-a. Asymmetric synthesis of phenylalanine using chiral phase transfer catalysts
1-b. Derivatization of citronellal
2-a. Cross-coupling reaction with Grignard reagents
2-b. Chemiluminescence with luminol
3. C-C bond formation with enolate anions
4. Lidocaine as a synthetic drug
5. Synthesis of benzene ring via [2+2+2] cycloisomerization of alkynes with ruthenium catalyst

* Advanced biotechnology
1. Elemental Experiments in Recombinant DNA Technology
2. Purification and Analysis of Protein
3a. Fluorescent DNA probe for detection of INDEL
3b. Microchip electrophoresis-based DNA analysis
4. Mammalian cell culture

•Textbook
Use an experimental guideline prepared for each topic. How to obtain the experimental guideline will be announced during the experiment guidance.

•Additional Reading
Vollhardt/Schore Organic Chemistry Structure and Function, 6th Ed.; K. P. C. Vollhardt, N. E. Schore
Biochemistry EIGHTH EDITION; J. M. Berg, J. L. Tymoczko, G.J. Gatto,Jr., L. Stryer

•Grade Assessment
Grading will be decided based on approach to the experiment (positiveness, activeness), logical thinking / judgment for the results, experimental skills and reports. Credits will be awarded to those students who score 60 or more out of 100 points.

•Notes
It is assumed that you have earned credits for Safety in Laboratory.

•Contacting Faculty
Professors and teaching assistants will answer the questions.

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