Chemistry and Biotechnology Laboratory 3(3.0 credits) | |||
| Code | : | 12443 | |
| Course Type | : | Specialized Courses | |
| Class Format | : | Experiment | |
| Course Name | : | Chemistry | |
| Starts 1 | : | 4 Autumn Semester | |
| Elective/Compulsory | : | Compulsory | |
| Lecturer | : | Faculty of Chemistry | |
| •Course Purpose |
| This course enhances the development of the student's skill in carrying out organic chemistry and biotechnology experiments. It will help your graduation research in 4th grade (graduation thesis A and B). Through organic chemistry laboratory, you will learn advanced experimental protocols for the synthesis, separation, purification, and identification of organic molecules. Biotechnology laboratory aims to learn advanced experimental procedure. |
| •Prerequisite Subjects |
| Elemental Chemistry I & II, Organic chemistry 1 ~ 5, Biochemistry 1, chemistry and biotechnology laboratory 2. |
| •Course Topics |
| * Advanced organic chemistry
1-a. Asymmetric synthesis of phenylalanine using chiral phase transfer catalysts 1-b. Derivatization of citronellal 2-a. Cross-coupling reaction with Grignard reagents 2-b. Chemiluminescence with luminol 3. C-C bond formation with enolate anions 4. Lidocaine as a synthetic drug 5. Synthesis of benzene ring via [2+2+2] cycloisomerization of alkynes with ruthenium catalyst * Advanced biotechnology 1. Elemental Experiments in Recombinant DNA Technology 2. Purification and Analysis of Protein 3a. Fluorescent DNA probe for detection of INDEL 3b. Microchip electrophoresis-based DNA analysis |
| •Textbook |
| Use an experimental guideline prepared for each topic. How to obtain the experimental guideline will be announced during the experiment guidance. |
| •Additional Reading |
| Vollhardt/Schore Organic Chemistry Structure and Function, 6th Ed.; K. P. C. Vollhardt, N. E. Schore
Biochemistry EIGHTH EDITION; J. M. Berg, J. L. Tymoczko, G.J. Gatto,Jr., L. Stryer |
| •Grade Assessment |
| Grading will be decided based on approach to the experiment (positiveness, activeness), logical thinking / judgment for the results, experimental skills and reports. Credits will be awarded to those students who score 60 or more out of 100 points. |
| •Notes |
| It is assumed that you have earned credits for Safety in Laboratory. |
| •Contacting Faculty |
| Professors and teaching assistants will answer the questions. Besides, students can make an appointment with the lecturers via email or NUCT.
Contact address: Hajime NAKATANI (nakatanih[at]chembio.nagoya-u.ac.jp) Muhammet UYANIK (muha[at]chembio.nagoya-u.ac.jp) |